Ovarian cancer is one of the most common and lethal types of gynecological malignancies worldwide, accounting for approximately 295,000 new cases and 185,000 deaths annually. The high lethality rate is due to multiple reasons, including recurrence and the resistance of recurrent tumors to chemotherapy. Cell line models are crucial for preclinical cancer studies, to identify mechanisms of disease, to study drug resistance, and to screen for candidate therapeutics.
Researchers at the National Cancer Institute (NCI), Laboratory of Cellular and Molecular Biology have derived a cell line, A2780, from a patient with metastatic ovarian adenocarcinoma who was not exposed to any anti-cancer agents before tumor extraction. This cell line forms tumors in nude mice and can be used to evaluate the effects of anti-cancer agents on ovarian cancer. Furthermore, cisplatin- (A2780CIS) and adriamycin- resistant (A2780ADR) derivatives have been developed by chronic exposure of the parental cell line to cisplatin and adriamycin, respectively. These lines show cross-resistance to other agents such as melphalan, vinblastine or irradiation, and can be used to study the molecular basis of drug resistance in ovarian cancer.
NCI is seeking parties to non-exclusively license these ovarian cancer cell lines.
- Research tool for drug screen and related preclinical studies of ovarian cancer therapeutics
- Research tool for mechanistic studies of ovarian cancer such as the basis of drug resistance
- Well-characterized ovarian cancer cell lines
- Parental A2780 may be used to generate other drug-resistant cell lines through exposure to low concentrations of other agents
Stuart Aaronson M.D. (NCI), Nelson W. Ellmore (NCI)
Eva A, et al. Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells. [PMID 6173755]
Westin EH, et al. Differential expression of the amv gene in human hematopoietic cells. [PMID 6954533]
Beaufort CM, et al. Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes. [PMID 25230021]
- Research Material: NIH will not pursue patent prosecution for this technology