Method for High-Throughput Microdissection and Analysis of Biological Samples

The National Cancer Institute's Laboratory of Pathology is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize a method for target-activated microdissection.

A variety of techniques have been used to micro-dissect specific cells or cell populations from a histological sample under direct microscopic visualization. Traditional  microdissection techniques involve painstaking manual dissection using needles or other micro-manipulation devices to isolate individual cells based on histologies.

This analysis tool performs specific target-activated transfer from a biological sample (i.e., tissue) that can be automated for high-throughput analysis.  The method employs a localized reagent, such as an absorbative stain, that allows micro-adhesion of desired cellular material in a tissue sample to a transfer surface such as a thermoplastic polymer film.  The energy from a light or heat source causes the adhesion of the target cells to the thermoplastic transfer surface.  The transfer surface is activated from within the target, for example, by heat. Subsequent separation of the film from the tissue section selectively removes the adhered target.  This in situ activation can be achieved by exposing the biological sample to an immunologic reagent that specifically binds to the target.

Further R&D Needed:

• Optimization of an instrument for target-activated microdissection.
• Evaluation of cellular targeting methodologies.